Goat anti-Human, Mouse NBEA Polyclonal Antibody | anti-NBEA antibody

NBEA (Neurobeachin, BCL8B, FLJ10197, KIAA1544, Lysosomal Trafficking Regulator 2, LYST2, Protein BCL8B, Protein Neurobeachin, RP11-270C18.1)

Cat. Num. AAA627168

• Gene Names: NBEA; BCL8B; LYST2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: NBEA; Polyclonal Antibody; NBEA (Neurobeachin; BCL8B; FLJ10197; KIAA1544; Lysosomal Trafficking Regulator 2; LYST2; Protein BCL8B; Protein Neurobeachin; RP11-270C18.1); Anti -NBEA (Neurobeachin; anti-NBEA antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NBEA. Species crossreactivity
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-NBEA antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:8000; Western Blot: 1-3ug/ml ~300kD band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327kD according to NP_056493.3). Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium.
• Immunogen: Synthetic peptide corresponding to C-DFNRWHYEHQNRY, from the C Terminus of human NBEA (NP_056493.3).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS627168 (1µg/ml) staining of 1) untransfected HEK293T cells 2) HEK293T cells transfected with mouse NBEA. Detected by chemiluminescence.)

Related Product Information for anti-NBEA antibody

This gene encodes a member of a large, diverse group of A-kinase anchor proteins that target the activity of protein kinase A to specific subcellular sites by binding to its type II regulatory subunits. Brain-specific expression and coat protein-like membrane recruitment of a highly similar protein in mouse suggest an involvement in neuronal post-Golgi membrane traffic. Mutations in this gene may be associated with a form of autism. Alternatively spliced transcript variants may exist, but their full-length nature has not been determined.

Product Categories/Family for anti-NBEA antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 323510703
• NCBI GeneID: 26960
• NCBI Accession #: NP_001191126.1
• NCBI GenBank Nucleotide #: NM_001204197.1
• UniProt Accession #: Q6N008; Q8NFP9
• Molecular Weight: 174,573 Da
• NCBI Official Full Name: neurobeachin isoform 2
• NCBI Official Synonym Full Names: neurobeachin
• NCBI Official Symbol: NBEA
• NCBI Official Synonym Symbols: BCL8B; LYST2
• NCBI Protein Information: neurobeachin; OTTHUMP00000018239; lysosomal-trafficking regulator 2
• UniProt Protein Name: Putative uncharacterized protein DKFZp686I03263
• Protein Family: Neurobeachin
• UniProt Gene Name: DKFZp686I03263
• UniProt Entry Name: Q6N008_HUMAN

NCBI Description

This gene encodes a member of a large, diverse group of A-kinase anchor proteins that target the activity of protein kinase A to specific subcellular sites by binding to its type II regulatory subunits. Brain-specific expression and coat protein-like membrane recruitment of a highly similar protein in mouse suggest an involvement in neuronal post-Golgi membrane traffic. Mutations in this gene may be associated with a form of autism. This gene and its expression are frequently disrupted in patients with multiple myeloma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants may exist, but their full-length nature has not been determined.

Uniprot Description

neurobeachin: Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins. Belongs to the WD repeat neurobeachin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 13q13

Cellular Component: extrinsic to membrane; plasma membrane; endomembrane system; trans-Golgi network; cytosol

Molecular Function: phospholipid binding; protein kinase binding

Biological Process: protein localization; endosome transport