Western Blot (WB)
((0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CTLA + CTLB Polyclonal Antibody | anti-CLTA antibody
Goat anti-CTLA + CTLB Antibody
Western Blot: Approx.35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol.2000 Sep;279(3): C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]
Uniprot Description
CLTA: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Belongs to the clathrin light chain family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Vesicle
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: clathrin coat; clathrin coat of coated pit; clathrin coat of trans-Golgi network vesicle; clathrin vesicle coat; cytoplasmic membrane-bound vesicle; cytosol; intracellular membrane-bound organelle; membrane; plasma membrane; trans-Golgi network membrane
Molecular Function: clathrin heavy chain binding; peptide binding; protein binding; structural molecule activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; intracellular protein transport; microtubule-based movement; negative regulation of epidermal growth factor receptor signaling pathway
Research Articles on CLTA
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Product Notes
The CLTA clta (Catalog #AAA421680) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CTLA + CTLB Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CTLA + CTLB can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx.35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol.2000 Sep;279(3): C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CLTA clta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CDFNPKSSKQ AKD. It is sometimes possible for the material contained within the vial of "CTLA + CTLB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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