Goat DLX5 Polyclonal Antibody | anti-DLX5 antibody

Goat anti-DLX5 Antibody

Cat. Num. AAA422203

• Gene Names: DLX5; SHFM1D
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: DLX5; Polyclonal Antibody; Goat anti-DLX5 Antibody; distal-less homeobox 5; distal-less homeo box 5; DLX5 antibody; distal-less homeobox 5 antibody; distal-less homeo box 5 antibody; anti-DLX5 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: AYNRVPSATNQPEK
• Sequence Length: 289

• Applicable Applications for anti-DLX5 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem.2003 Sep 5;278(36): 34387-94; PMID: 12815054). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-AYNRVPSATNQPEK, from the internal region of the protein sequence according to NP_005212.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885187
• NCBI GeneID: 1749
• NCBI Accession #: NP_005212.1
• NCBI GenBank Nucleotide #: NM_005221.5
• Molecular Weight: 20,900 Da
• NCBI Official Full Name: homeobox protein DLX-5
• NCBI Official Synonym Full Names: distal-less homeobox 5
• NCBI Official Symbol: DLX5
• NCBI Official Synonym Symbols: SHFM1D
• NCBI Protein Information: homeobox protein DLX-5
• UniProt Protein Name: Homeobox protein DLX-5
• Protein Family: Homeobox protein
• UniProt Gene Name: DLX5
• UniProt Entry Name: DLX5_HUMAN

NCBI Description

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq, Jul 2008]

Uniprot Description

DLX5: Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding. Defects in DLX5 are the cause of split-hand/foot malformation type 1, with sensorineural hearing loss (SHFM1D). A disease characterized by the association of split- hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Belongs to the distal-less homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytoplasm; nuclear chromatin

Biological Process: anatomical structure formation; axon guidance; BMP signaling pathway; cell proliferation; embryonic limb morphogenesis; endochondral ossification; epithelial cell differentiation; inner ear morphogenesis; nervous system development; olfactory pit development; osteoblast differentiation; palate development; positive regulation of epithelial cell proliferation; positive regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; skeletal development; transcription from RNA polymerase II promoter

Disease: Split-hand/foot Malformation 1 With Sensorineural Hearing Loss, Autosomal Recessive