Mouse Choline O-acetyltransferase ELISA Kit | Chat elisa kit

Mouse Choline O-acetyltransferase ELISA Kit

Cat. Num. AAA760409

• Reactivity: Mouse
• Synonyms: Choline O-acetyltransferase; Mouse Choline O-acetyltransferase ELISA Kit; Chat/Choline O-acetyltransferase; Chat elisa kit

• Reactivity: Mouse
• Sequence Length: 644

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Chat elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Chat antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Chat antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Chat amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Chat can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 282154809
• NCBI GeneID: 290567
• NCBI Accession #: NP_001164064.1
• NCBI GenBank Nucleotide #: NM_001170593.1
• Molecular Weight: 71,864 Da
• NCBI Official Full Name: choline O-acetyltransferase
• NCBI Official Synonym Full Names: choline O-acetyltransferase
• NCBI Official Symbol: Chat
• NCBI Protein Information: choline O-acetyltransferase
• UniProt Protein Name: Choline O-acetyltransferase
• Protein Family: Choline O-acetyltransferase
• UniProt Gene Name: Chat
• UniProt Synonym Gene Names: CHOACTase; ChAT; Choline acetylase
• UniProt Entry Name: CLAT_RAT

Uniprot Description

CHAT: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses

Protein type: Lipid Metabolism - glycerophospholipid; EC 2.3.1.6; Acetyltransferase

Cellular Component: axon; cell soma; cytoplasm; mitochondrion; neuron projection

Molecular Function: choline binding; choline O-acetyltransferase activity; transferase activity, transferring acyl groups

Biological Process: acetylcholine biosynthetic process; adult walking behavior; antral ovarian follicle growth; dendrite development; establishment of synaptic specificity at neuromuscular junction; memory; muscle development; neuromuscular synaptic transmission; neuron differentiation; neurotransmitter biosynthetic process; response to drug; response to ethanol; response to hypoxia; response to nutrient; rhythmic behavior; rhythmic excitation; synaptic transmission