Rabbit anti-Human NANOS1 Polyclonal Antibody | anti-NANOS1 antibody

Anti-NANOS1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100463

• Gene Names: NANOS1; NOS1; SPGF12
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NANOS1; Polyclonal Antibody; Anti-NANOS1 Antibody; Rabbit Polyclonal; NANOS1 Antibody; Rabbit PAb; Antigen Affinity Purified; nanos homolog 1 (Drosophila); Anti-EC_Rep1a Antibody; Anti-NOS-1 Antibody; Anti-NOS1 Antibody; Anti-RP11-498J9.3 Antibody; Anti-SPGF12 Antibody; anti-NANOS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NANOS1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NANOS1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human NANOS1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human NANOS1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NANOS1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:36 kDa)

Related Product Information for anti-NANOS1 antibody

The Nanos gene is a key translational regulator of specific mRNAs involved in Drosophila germ cell development. A highly conserved human protein PUMILIO2 forms a complex with NANOS1 in human male germ cells, as does the Drosophila ancestor Pumilio, which binds Nanos to regulate translation of specific mRNAs. NANOS1-PUMILIO2 complex, together with GEMIN3 and small non-coding RNAs, possibly regulate mRNA translation within CB of the human germ cells.

NCBI and Uniprot Product Information

• NCBI GI #: 41688589
• NCBI GeneID: 340719
• UniProt Accession #: Q8WY41
• Molecular Weight: 30,230 Da
• NCBI Official Full Name: Nanos homolog 1
• NCBI Official Synonym Full Names: nanos homolog 1 (Drosophila)
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; SPGF12
• NCBI Protein Information: nanos homolog 1; NOS-1; EC_Rep1a
• UniProt Protein Name: Nanos homolog 1
• Protein Family: Nanos
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; translation repressor activity; zinc ion binding; RNA binding

Biological Process: cell migration; negative regulation of translation

Disease: Spermatogenic Failure 12