Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ALDH9A1 Polyclonal Antibody | anti-ALDH9A1 antibody
Goat anti-ALDH9A1 Antibody
Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of a few cells between the Granular Cell layer and the Molecular Layer of the Cerebellum. Please note the unstained Purkinje cell at 10 O'clock. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 50kDa band observed in Human Kidney and Liver lysates (calculated MW of 56.3kDa according to NP_000687.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits. [provided by RefSeq, Jul 2008]
Uniprot Description
ALDH9A1: Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction. Belongs to the aldehyde dehydrogenase family.
Protein type: Amino Acid Metabolism - histidine; EC 1.2.1.3; Amino Acid Metabolism - arginine and proline; Oxidoreductase; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Secondary Metabolites Metabolism - limonene and pinene degradation; Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid; EC 1.2.1.47; Mitochondrial; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - pyruvate; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tryptophan
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: cytoplasm; cytosol
Molecular Function: 1-pyrroline dehydrogenase activity; 3-chloroallyl aldehyde dehydrogenase activity; 4-trimethylammoniobutyraldehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; amine binding; aminobutyraldehyde dehydrogenase activity; NAD binding; protein homodimerization activity
Biological Process: aldehyde metabolic process; carnitine biosynthetic process; hormone metabolic process; kidney development; liver development; neurotransmitter biosynthetic process
Research Articles on ALDH9A1
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Product Notes
The ALDH9A1 aldh9a1 (Catalog #AAA422398) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ALDH9A1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH9A1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of a few cells between the Granular Cell layer and the Molecular Layer of the Cerebellum. Please note the unstained Purkinje cell at 10 O'clock. Recommended concentration, 3-5ug/ml. Western Blot: Approx 50kDa band observed in Human Kidney and Liver lysates (calculated MW of 56.3kDa according to NP_000687.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the ALDH9A1 aldh9a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QKEILDKFTE EVVKQ. It is sometimes possible for the material contained within the vial of "ALDH9A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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