Rabbit N/H/K-Ras Polyclonal Antibody | anti-N/H/K-Ras antibody

N/H/K-Ras Polyclonal Antibody

Cat. Num. AAA9700697

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: N/H/K-Ras; Polyclonal Antibody; N/H/K-Ras Polyclonal Antibody; Rabbit Anti-N/H/K-Ras Polyclonal Antibody; NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; anti-N/H/K-Ras antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-N/H/K-Ras antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300; ELISA: 1:20000
• Immunogen: Synthesized peptide derived from the N-terminal region of human N/H/K-Ras at AA range: 10-90
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of L929 (1), Rat-musle (2), diluted at 1:1000.)

Related Product Information for anti-N/H/K-Ras antibody

HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. GTPase Hras undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NP_002515.1
• UniProt Accession #: P01111; P01112; P01116
• Molecular Weight: 21 KD
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: NRAS proto-oncogene, GTPase
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas
• UniProt Protein Name: GTPase NRas
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]