Rabbit Ras Polyclonal Antibody | anti-Ras antibody

Ras Polyclonal Antibody

Cat. Num. AAA3013348

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Ras; Polyclonal Antibody; Ras Polyclonal Antibody; NRAS; HRAS; KRAS; C-BAS/HAS; c-H-ras; C-HA-RAS1; CTLO; GTPase HRas; GTPase KRas; GTPase NRas; H-Ras-1; H-RASIDX; Ha-Ras; HAMSV; HRAS1; K RAS2A; K RAS2B; K RAS4A; K RAS4B; K-RAS; KRAS1; KRAS2; N-RAS; N-terminally processed; NRAS1; p21ras; RASH; RASH1; RASK2; Transforming protein p21; v Ha ras Harvey rat sarcoma viral oncogene homolog; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog; v ras neuroblastoma RAS viral oncogene homolog; anti-Ras antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ras polyclonal antibody detects endogenous levels of Ras protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 189

• Applicable Applications for anti-Ras antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:2,000; ICC: 1:100-1:500
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: MCF-7 Lane 3: Hela Lane 4: zebrafish)

Immunocytochemistry (ICC)

(ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Ras antibody

The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NP_002515.1
• UniProt Accession #: P01111; P01112; P01116; P08556; P32883; Q61411; P08644; P20171; Q04970
• Molecular Weight: 21kDa
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: NRAS proto-oncogene, GTPase
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas
• UniProt Protein Name: GTPase NRas
• Protein Family: Ras protein
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]