ING5 recombinant protein

ING5 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121581

• Gene Names: ING5; p28ING5
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ING5; ING5 Protein; Human; Recombinant (GST Tag); Human ING5 Protein (GST Tag); p28ING5 Protein; inhibitor of growth family; member 5; ING5 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu36-Gln84

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human ING5 (Q8WYH8-2)(Glu36-Gln84) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ING5 recombinant protein

Background: ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16, and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6, and one of BRPF1, BRD1/BRPF2, and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation, ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyltransferase complex, suggesting its involvement in the TP53-dependent regulatory pathway.

References

Zhang F, et al. (2011) The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects. PLoS One. (7):e21505.Zheng HC, et al. (2011) The nuclear to cytoplasmic shift of ING5 protein during colorectal carcinogenesis with their distinct links to pathologic behaviors of carcinomas. Hum Pathol. 42(3):424-33.Xing YN, et al. (2011) The altered expression of ING5 protein is involved in gastric carcinogenesis and subsequent progression. Hum Pathol. 42(1):25-35.

NCBI and Uniprot Product Information

• NCBI GI #: 57012960
• NCBI GeneID: 84289
• UniProt Accession #: Q8WYH8; Q53NU6; Q57Z54; Q9BS30; A8K1P3
• Molecular Weight: 240
• NCBI Official Full Name: Inhibitor of growth protein 5
• NCBI Official Synonym Full Names: inhibitor of growth family, member 5
• NCBI Official Symbol: ING5
• NCBI Official Synonym Symbols: p28ING5
• NCBI Protein Information: inhibitor of growth protein 5
• UniProt Protein Name: Inhibitor of growth protein 5
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING5
• UniProt Entry Name: ING5_HUMAN

NCBI Description

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ING5: Component of the HBO1 complex which has a histone H4- specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. Belongs to the ING family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; chromatin binding; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; protein amino acid acetylation; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; DNA replication; negative regulation of growth