Rabbit Ras Monoclonal Antibody | anti-Ras antibody

Ras Rabbit Monoclonal Antibody

Cat. Num. AAA3014038

• Gene Names: NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• Reactivity: Human, Mouse, Rat, Zebra fish
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: Ras; Monoclonal Antibody; Ras Rabbit Monoclonal Antibody; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; NRAS; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; KRAS; KRAS2; RASK2; anti-Ras antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebra fish
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody is predicted to react with H-Ras, N-Ras and K-Ras.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 189

• Applicable Applications for anti-Ras antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 1:50-1:200; FC/FACS: 1:10-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ras Rabbit mAb at 1:1000 dilutionLane1:MCF7 whole cell lysateLane2:293T whole cell lysateLane3:The brain tissue lysate of Mouse)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-Ras antibody

Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4505451
• NCBI GeneID: 4893
• NCBI Accession #: NP_002515.1
• NCBI GenBank Nucleotide #: NP_002515.1
• UniProt Accession #: P01111; P01112; P01116
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: GTPase NRas
• NCBI Official Synonym Full Names: NRAS proto-oncogene, GTPase
• NCBI Official Symbol: NRAS
• NCBI Official Synonym Symbols: NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
• NCBI Protein Information: GTPase NRas
• UniProt Protein Name: GTPase NRas
• Protein Family: Ras protein
• UniProt Gene Name: NRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASN_HUMAN

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]