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Western Blot (WB) (Western BlotPositive WB detected in: Mouse brain tissueAll lanes: MYRF antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 125, 121 KDaObserved band size: 125 KDa)

Rabbit anti-Human, Mouse MYRF Polyclonal Antibody | anti-MYRF antibody

MYRF Antibody

Gene Names
MYRF; MRF; Ndt80; pqn-47; C11orf9
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
MYRF; Polyclonal Antibody; MYRF Antibody; Myelin regulatory factor; Myelin gene regulatory factor; N-terminal; C-terminal; C11orf9; KIAA0954; MRF; anti-MYRF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Sequence Length
1151
Applicable Applications for anti-MYRF antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
Immunogen
Recombinant Human Myelin regulatory factor protein (843-1004AA)
Conjugation
Non-conjugated
Species
Human
Research Area
Others
Target
Names MYRF
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: MYRF antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 125, 121 KDaObserved band size: 125 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Mouse brain tissueAll lanes: MYRF antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 125, 121 KDaObserved band size: 125 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung tissue using MBS7104353 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung tissue using MBS7104353 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with MBS7104353 at 1:133, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of A549 cells with MBS7104353 at 1:133, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for anti-MYRF antibody
Myelin regulatory factor: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes (PubMed:23966832).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
745
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120,225 Da
NCBI Official Full Name
myelin regulatory factor isoform 2
NCBI Official Synonym Full Names
myelin regulatory factor
NCBI Official Symbol
MYRF
NCBI Official Synonym Symbols
MRF; Ndt80; pqn-47; C11orf9
NCBI Protein Information
myelin regulatory factor
UniProt Protein Name
Myelin regulatory factor
Protein Family
UniProt Gene Name
MYRF
UniProt Synonym Gene Names
C11orf9; KIAA0954; MRF

NCBI Description

This gene encodes a transcription factor that is required for central nervous system myelination and may regulate oligodendrocyte differentiation. It is thought to act by increasing the expression of genes that effect myelin production but may also directly promote myelin gene expression. Loss of a similar gene in mouse models results in severe demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

Myelin regulatory factor: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes (PubMed:23966832).

Research Articles on MYRF

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Product Notes

The MYRF myrf (Catalog #AAA7104353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYRF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYRF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the MYRF myrf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYRF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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