Western Blot (WB)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: lexA antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 39 KDaobserved band size: 39 KDa)
Rabbit lexA Polyclonal Antibody | anti-lexA antibody
lexA Antibody
Reactivity
Escherichia coli
Applications
ELISA, Western Blot
Purity
>95%, Protein G purified
Synonyms
lexA; Polyclonal Antibody; lexA Antibody; LexA repressor; anti-lexA antibody
Host
Rabbit
Reactivity
Escherichia coli
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
202
Applicable Applications for anti-lexA antibody
ELISA (EIA); Western Blot (WB)
Application Notes
WB:1:500-1:5000
Immunogen
Recombinant Escherichia coli LexA repressor protein (1-202AA)
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Species
Escherichia coli
Research Area
Others
Target Names
lexA
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: lexA antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 39 KDaobserved band size: 39 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: lexA antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 39 KDaobserved band size: 39 KDa)
Related Product Information for anti-lexA antibody
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed: 20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed: 22412352).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transcriptional repressor of SOS regulon
UniProt Protein Name
LexA repressor
Protein Family
UniProt Gene Name
lexA
UniProt Entry Name
LEXA_ECOLI
Uniprot Description
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
Similar Products
Product Notes
The lexA lexa (Catalog #AAA7049963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The lexA Antibody reacts with Escherichia coli and may cross-react with other species as described in the data sheet. AAA Biotech's lexA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA); Western Blot (WB). WB:1:500-1:5000. Researchers should empirically determine the suitability of the lexA lexa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "lexA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
