Rabbit ROBO3 Polyclonal Antibody | anti-ROBO3 antibody

ROBO3 Polyclonal Antibody

Cat. Num. AAA2527884

• Gene Names: ROBO3; HGPS; RIG1; HGPPS; RBIG1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: ROBO3; Polyclonal Antibody; ROBO3 Polyclonal Antibody; HGPS; RIG1; HGPPS; RBIG1; anti-ROBO3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.9mg/mL (varies by lot)
• Sequence Length: 1386

• Applicable Applications for anti-ROBO3 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:25-1:100
• Immunogen: Recombinant protein of human ROBO3
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human breast cancer tissue using ROBO3 Polyclonal Antibody at dilution 1:35)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using ROBO3 Polyclonal Antibody at dilution 1:35)

Related Product Information for anti-ROBO3 antibody

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.

NCBI and Uniprot Product Information

• NCBI GI #: 48476182
• NCBI GeneID: 64221
• NCBI Accession #: NP_071765.2
• NCBI GenBank Nucleotide #: NM_022370.3
• Molecular Weight: 110,676 Da
• NCBI Official Full Name: roundabout homolog 3
• NCBI Official Synonym Full Names: roundabout, axon guidance receptor, homolog 3 (Drosophila)
• NCBI Official Symbol: ROBO3
• NCBI Official Synonym Symbols: HGPS; RIG1; HGPPS; RBIG1
• NCBI Protein Information: roundabout homolog 3; retinoblastoma inhibiting gene 1; roundabout-like protein 3
• UniProt Protein Name: Roundabout homolog 3
• Protein Family: Roundabout
• UniProt Gene Name: ROBO3
• UniProt Entry Name: ROBO3_HUMAN

NCBI Description

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]

Uniprot Description

ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: axon; integral to membrane

Biological Process: axon guidance; neuron migration; axon midline choice point recognition

Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis