Rabbit anti-Human MYBL2/B-MyB Polyclonal Antibody | anti-MYBL2 antibody

Anti-MYBL2/B-MyB Antibody, Rabbit Polyclonal

Cat. Num. AAA8109140

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MYBL2/B-MyB; Polyclonal Antibody; Anti-MYBL2/B-MyB Antibody; Rabbit Polyclonal; MYBL2/B-MyB Antibody; Rabbit PAb; Antigen Affinity Purified; v-myb avian myeloblastosis viral oncogene homolog-like 2; Anti-B-MYB Antibody; Anti-BMYB Antibody; anti-MYBL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MYBL2/B-MyB
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MYBL2 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human MYBL2/B-MyB fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MYBL2/B-MyB fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MYBL2 in human adrenal gland with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MYBL2 in human bronchus with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MYBL2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYBL2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.)

Related Product Information for anti-MYBL2 antibody

MYBL2 (B-MYB), a member of the MYB family of transcription factor genes, regulates the expression of genes in the process of tumorigenesis. Many studies have shown that MYBL2 is highly expressed in several human malignancies including pancreatic ductal adenocarcinoma (PDAC). Overexpression of MYBL2 might serve as a novel prognostic biomarker in PDAC patients. MYBL2 is a key downstream factor of Akt/FoxM1 signaling to promote the progression of human glioma and could be a new candidate gene for molecular targeting therapy and biomarker for radiotherapy of glioma. MYBL2 (alias B-Myb) is a physiological regulator of cell cycle progression, cell survival, and cell differentiation. When deregulated in cancer cells, MYBL2 mediates the deregulation of these properties. MYBL2 and players of its downstream transcriptional network can be used as prognostic and/or predictive biomarkers as well as potential therapeutic targets to offer less toxic and more specific anti-cancer therapies in the future.

References

Zhang X, et al. (2017) Akt/foxm1 signaling pathway-mediated upregulation of mybl2 promotes progression of human glioma. J Exp Clin Cancer Res 36 (1): 105.Yu R, et al. (2017) Clinicopathologic features and prognostic implications of mybl2 protein expression in pancreatic ductal adenocarcinoma. Pathol Res Pract 213 (8): 964-968.Musa J, et al. (2017) Mybl2 (b-myb): A central regulator of cell proliferation, cell survival and differentiation involved in tumorigenesis. Cell Death Dis 8 (6): e2895.