Immunohistochemistry (IHC)
(Immunochemical staining of human SRCIN1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human SRCIN1 Polyclonal Antibody | anti-SRCIN1 antibody
Anti-SRCIN1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SRCIN1; Polyclonal Antibody; Anti-SRCIN1 Antibody; Rabbit Polyclonal; SRCIN1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-bHLHd2 Antibody; Anti-P140 Antibody; Anti-p140Cap Antibody; Anti-SNIP Antibody; Anti-SREBP-2 Antibody; Anti-SREBP2 Antibody; anti-SRCIN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SRCIN1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SRCIN1 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human SRCIN1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SRCIN1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human SRCIN1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SRCIN1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of SRCIN1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SRCIN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of SRCIN1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SRCIN1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Related Product Information for anti-SRCIN1 antibody
SRCIN1 (SRC kinase signalling inhibitor 1), also named as p140 Cas-associated protein (p140CAP), contains two regions of highly charged amino acids, two proline-rich regions and two coiled-coil domains. SRCIN1 functions as a tumour suppressor gene and may slow tumour progression. SRCIN1 acts as a regulator for inhibiting cell spreading and migration and is also involved in calcium-dependent exocytosis. SRCIN1 is identified to be an inhibitor in lung cancer and breast cancer along with osteosarcoma. SRCIN1is involved in calcium-dependent exocytosis. SRCIN1 may play a role in neurotransmitter release or synapse maintenance.
References
Zhang M, et al. (2017) Overexpression of srcin1 contributes to the growth and metastasis of colorectal cancer. Int J Oncol 50 (5): 1555-1566.Wang P, et al. (2016) Srcin1 suppressed osteosarcoma cell proliferation and invasion. PLoS One 11 (8): e0155518.Hong W, et al. (2020) Srcin1 regulated by circccdc66/mir-211 is upregulated and promotes cell proliferation in non-small-cell lung cancer. Biomed Res Int 2020 5307641.
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Product Notes
The SRCIN1 (Catalog #AAA8110088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SRCIN1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SRCIN1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:50-1:200 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the SRCIN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRCIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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