Western Blot (WB)
(Western Blot analysis of various cells with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:1000)
Rabbit mTOR Polyclonal Antibody | anti-mTOR antibody
Phospho-mTOR (S2448) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity purification
Synonyms
mTOR; Polyclonal Antibody; Phospho-mTOR (S2448) Polyclonal Antibody; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; Mechanistic tar; anti-mTOR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4.
Concentration
1mg/ml (varies by lot)
Sequence Length
168
Applicable Applications for anti-mTOR antibody
Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:40000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:40000
Immunogen
Synthesized peptide derived from human mTOR around the phosphorylation site of S2448.
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of various cells with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of various cells with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse kidney tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse kidney tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)
Immunofluorescence (IF)
(Immunofluorescence analysis of Rat lung tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)
Immunofluorescence (IF)
(Immunofluorescence analysis of Rat lung tissue with Phospho-mTOR (Ser2448) Polyclonal Antibody at dilution of 1:200)
Related Product Information for anti-mTOR antibody
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. The antibody is specific to MTOR.
Product Categories/Family for anti-mTOR antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Calculated MW: 289kDa
Observed MW: 289kDa
Observed MW: 289kDa
NCBI Official Full Name
MTOR, partial
UniProt Protein Name
MTOR
Protein Family
UniProt Gene Name
MTOR
UniProt Entry Name
A0A0S7M2X4_9TELE
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Product Notes
The mTOR mtor (Catalog #AAA2537730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-mTOR (S2448) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mTOR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:40000. Researchers should empirically determine the suitability of the mTOR mtor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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