Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EVL Polyclonal Antibody | anti-EVL antibody
Goat anti-EVL Antibody
Western Blot: Approx.50kDa band observed in lysates of cell line Jurkat (calculated MW of 44.8kDa according to NP_057421.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
EVL: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. Required to transform actin polymerization into active movement for the propulsive force of Listeria monocytogenes. Belongs to the Ena/VASP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q32.2
Cellular Component: cytoplasm; cytoskeleton; cytosol; focal adhesion; lamellipodium; membrane
Molecular Function: actin binding; profilin binding; protein binding; SH3 domain binding
Biological Process: actin filament organization; actin polymerization and/or depolymerization; axon guidance; cell surface receptor linked signal transduction; nervous system development; organ morphogenesis; positive regulation of actin filament polymerization; positive regulation of stress fiber formation; protein homotetramerization
Research Articles on EVL
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Product Notes
The EVL evl (Catalog #AAA421775) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EVL Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EVL can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.50kDa band observed in lysates of cell line Jurkat (calculated MW of 44.8kDa according to NP_057421.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the EVL evl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQPPNSSEAG RKP. It is sometimes possible for the material contained within the vial of "EVL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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