Testing Data
Rabbit EWSR Polyclonal Antibody | anti-EWSR antibody
Rabbit anti EWSR1 Polyclonal antibody
Reactivity
Human, Rat, Mouse, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
EWSR; Polyclonal Antibody; Rabbit anti EWSR1 Polyclonal antibody; Rabbit anti-EWSR1; Ewing sarcoma breakpoint region 1; EWS; EWSR1; EWSR-1.; anti-EWSR antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Canine
Clonality
Polyclonal
Specificity
This antibody recognizes ~70 kDa of EWSR1 protein. It also cross reacts with human, mouse and rat tissues. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-EWSR antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the internal segment of EWSR1 protein. This sequence is identical among human, rat, mouse and dog species.
Positive Control
Mouse brain tissue
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-EWSR antibody
The EWSR1 gene encodes a putative RNA binding protein. Mutation in this gene causes Ewing sarcoma and neuroectodermal and various other tumors. This gene is involved in a number of different sarcomas as a result of chromosomal translocations. EWSR1 is fused to an ETS transcription factor (FLI-1, ERG, ETV-1, E1AF or FEV) in Ewing sarcoma; to activating transcription factor-1 (ATF-1) in soft tissue clear cell sarcoma; to Wilms Tumor -1 (WT1) in desmoplastic small round cell; to nuclear receptor 4A3 (NR4A3) in extraskeletal myxoid chondrosarcoma, and to C/EBPhomologous protein (CHOP) in myxoid liposarcoma. EWSR1 genes are required for mitotic integrity and survival of neural cells in the early embryonic development, and that the involvement of EWSR1 in mitotic stability is conserved from zebrafish
References
Melot,T., et al. Characterization of a new brain-specific isoform of the EWS oncoprotein. Eur. J. Biochem. 268 (12), 3483-3489 (2001)
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Product Notes
The EWSR (Catalog #AAA462243) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti EWSR1 Polyclonal antibody reacts with Human, Rat, Mouse, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's EWSR can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the EWSR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EWSR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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