Western Blot (WB)
(Western blot analysis of MRCKG expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse MRCKG Polyclonal Antibody | anti-MRCKG antibody
MRCKG Antibody
Gene Names
CDC42BPG; DMPK2; MRCKG; HSMDPKIN; KAPPA-200; MRCKgamma
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MRCKG; Polyclonal Antibody; MRCKG Antibody; CDC42 binding protein kinase gamma; DMPK like gamma; DMPK2; MRCK gamma; Myotonic dystrophy kinase related CDC42 binding kinase gamma; Myotonic dystrophy protein kinase like alpha; Serine/threonine protein kinase MRCK gamma; anti-MRCKG antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MRCKG antibody detects endogenous levels of total MRCKG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1551
Applicable Applications for anti-MRCKG antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Note: concentrates at the leading edge of cells.
Tissue Specificity
Expressed in heart and skeletal muscle.
Predicted Cross Reactivity
Sheep
Similarity
Sheep (82%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of MRCKG expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MRCKG expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9603927 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603927 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MRCKG antibody
Function: May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).
Subunit Structure: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Subunit Structure: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 173 kDa
NCBI Official Full Name
serine/threonine-protein kinase MRCK gamma
NCBI Official Synonym Full Names
CDC42 binding protein kinase gamma
NCBI Official Symbol
CDC42BPG
NCBI Official Synonym Symbols
DMPK2; MRCKG; HSMDPKIN; KAPPA-200; MRCKgamma
NCBI Protein Information
serine/threonine-protein kinase MRCK gamma
UniProt Protein Name
Serine/threonine-protein kinase MRCK gamma
UniProt Gene Name
CDC42BPG
UniProt Synonym Gene Names
MRCK gamma; MRCKG; Myotonic dystrophy protein kinase-like gamma
Uniprot Description
May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation ().
Research Articles on MRCKG
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Product Notes
The MRCKG cdc42bpg (Catalog #AAA9603927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRCKG Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRCKG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MRCKG cdc42bpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRCKG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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