Rabbit anti-Human ZO-1 Polyclonal Antibody | anti-ZO-1 antibody

ZO-1 antibody

Cat. Num. AAA9408542

• Gene Names: TJP1; ZO-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: ZO-1; Polyclonal Antibody; ZO-1 antibody; anti-ZO-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 1668

• Applicable Applications for anti-ZO-1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 266 of Human TJP1
• Target Name: ZO-1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample(30 ug whole cell lysate)A: H12995% SDS PAGEPrimary antibody diluted at 1: 3000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1 antibody at 1: 250 dilution.)

Related Product Information for anti-ZO-1 antibody

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Product Categories/Family for anti-ZO-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 116875765
• NCBI GeneID: 7082
• NCBI Accession #: NP_783297
• NCBI GenBank Nucleotide #: NM_175610.3
• Molecular Weight: 186,966 Da
• NCBI Official Full Name: tight junction protein ZO-1 isoform b
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: TJP1
• NCBI Official Synonym Symbols: ZO-1
• NCBI Protein Information: tight junction protein ZO-1
• UniProt Protein Name: Tight junction protein ZO-1
• UniProt Gene Name: TJP1
• UniProt Synonym Gene Names: ZO1
• UniProt Entry Name: ZO1_HUMAN

NCBI Description

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: apical junction complex; intercellular canaliculus; tight junction; apicolateral plasma membrane; basolateral plasma membrane; cytosol; cell-cell adherens junction; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; gap junction; cell junction; nucleus

Molecular Function: protein C-terminus binding; calmodulin binding; protein domain specific binding; protein binding

Biological Process: response to drug; intercellular junction assembly; response to ethanol; sensory perception of sound; apoptosis; negative regulation of vascular permeability; blastocyst formation; response to lipopolysaccharide; cell structure disassembly during apoptosis