Chicken ALDOA ELISA Kit | ALDOA elisa kit

Chicken ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit

Cat. Num. AAA2514203

• Gene Names: ALDOA; ALDA; GSD12; HEL-S-87p
• Reactivity: Chicken
• Synonyms: ALDOA; Chicken ALDOA (Aldolase A; Fructose Bisphosphate) ELISA Kit; ALDOA elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes Chicken ALDOA in samples. No significant cross-reactivity or interference between Chicken ALDOA and analogues was observed.
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.125-200pg/mL
• Sensitivity: 1.875pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Chicken ALDOA were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Chicken ALDOA were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALDOA elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken ALDOA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken ALDOA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken ALDOA and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken ALDOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Chicken ALDOA. You can calculate the concentration of Chicken ALDOA in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49456715
• NCBI GeneID: 226
• UniProt Accession #: P04075; Q6FH76; Q6FI10; Q96B15; Q9BWD9; Q9UCN2; B4DXI7
• Molecular Weight: 45,261 Da
• NCBI Official Full Name: ALDOA, partial
• NCBI Official Synonym Full Names: aldolase A, fructose-bisphosphate
• NCBI Official Symbol: ALDOA
• NCBI Official Synonym Symbols: ALDA; GSD12; HEL-S-87p
• NCBI Protein Information: fructose-bisphosphate aldolase A; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; lung cancer antigen NY-LU-1; muscle-type aldolase
• UniProt Protein Name: Fructose-bisphosphate aldolase A
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOA
• UniProt Synonym Gene Names: ALDA
• UniProt Entry Name: ALDOA_HUMAN

NCBI Description

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

Uniprot Description

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Protein type: Mitochondrial; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; Lyase; EC 4.1.2.13

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: I band; extracellular space; membrane; extracellular region; M band; nucleus; cytosol; actin cytoskeleton

Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding

Biological Process: platelet activation; striated muscle contraction; glycolysis; glucose metabolic process; actin filament organization; pathogenesis; gluconeogenesis; protein homotetramerization; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process

Disease: Glycogen Storage Disease Xii