Western Blot (WB)
((0. 02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MOB2 Polyclonal Antibody | anti-MOB2 antibody
Goat anti-MOB2 (aa128-140) Antibody
Gene Names
MOB2; HCCA2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MOB2; Polyclonal Antibody; Goat anti-MOB2 (aa128-140) Antibody; HCCA2; HCCA2 protein; mob2 homolog; mps one binder kinase activator-like 2; MOB2 antibody; HCCA2 antibody; HCCA2 protein antibody; mob2 homolog antibody; mps one binder kinase activator-like 2 antibody; MOB2 (aa128-140); anti-MOB2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DERGKKVKCTAPQ
Sequence Length
268
Applicable Applications for anti-MOB2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10ug/ml.
Immunohistochemistry: In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10ug/ml.
Immunogen
Peptide with sequence DERGKKVKCTAPQ, from the internal region of the protein sequence according to NP_001165694.1; NP_443731.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,174 Da
NCBI Official Full Name
MOB kinase activator 2 isoform 1
NCBI Official Synonym Full Names
MOB kinase activator 2
NCBI Official Symbol
MOB2
NCBI Official Synonym Symbols
HCCA2
NCBI Protein Information
MOB kinase activator 2
UniProt Protein Name
MOB kinase activator 2
Protein Family
UniProt Gene Name
MOB2
UniProt Synonym Gene Names
HCCA2
UniProt Entry Name
MOB2_HUMAN
Uniprot Description
HCCA2: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; nucleolus; nucleus; perinuclear region of cytoplasm
Molecular Function: metal ion binding
Biological Process: actin cytoskeleton organization and biogenesis; positive regulation of protein amino acid phosphorylation
Research Articles on MOB2
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Product Notes
The MOB2 mob2 (Catalog #AAA422882) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MOB2 (aa128-140) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MOB2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10ug/ml. Researchers should empirically determine the suitability of the MOB2 mob2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DERGKKVKCT APQ. It is sometimes possible for the material contained within the vial of "MOB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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