Goat MOB2 Polyclonal Antibody | anti-MOB2 antibody

Goat Polyclonal to Human MOB2

Cat. Num. AAA248655

• Gene Names: MOB2; HCCA2
• Reactivity: Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MOB2; Polyclonal Antibody; Goat Polyclonal to Human MOB2; Anti-MOB2 Antibody (Internal) IHC-plus; HCCA2; MOB kinase activator 2; MOB2 Mps One Binder homolog; Mob2 homolog; Human MOB2; anti-MOB2 antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human MOB2 / HCCA2. This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-MOB2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.02 - 0.06 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line Jurkat (calculated MW of 30.2kD according to NP_001165694.1). Recommended concentration: 0.02-0.06 ug/ml. An additional band of...
• Target Species: Human
• Immunogen Description: Synthetic peptide DERGKKVKCTAPQ from an internal region of human MOB2 / HCCA2 (NP_001165694.1; NP_443731.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Xenopus (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: MOB2 antibody was raised against synthetic peptide DERGKKVKCTAPQ from an internal region of human MOB2 / HCCA2 (NP_001165694.1; NP_443731.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Xenopus (100%); Chicken, Zebrafish (92%); Smelt, Seq squirt (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MOB2 antibody IHC staining of human lung, airway. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(MOB2 antibody (0.02 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 288557345
• NCBI GeneID: 81532
• NCBI Accession #: NP_001165694.1
• NCBI GenBank Nucleotide #: NM_001172223.2
• UniProt Accession #: Q70IA6; Q96M67; B4DKP3
• Molecular Weight: 30,174 Da
• NCBI Official Full Name: MOB kinase activator 2 isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 2
• NCBI Official Symbol: MOB2
• NCBI Official Synonym Symbols: HCCA2
• NCBI Protein Information: MOB kinase activator 2; MOB2 Mps One Binder homolog; Mps one binder kinase activator-like 2
• UniProt Protein Name: MOB kinase activator 2
• Protein Family: MOB kinase activator
• UniProt Gene Name: MOB2
• UniProt Synonym Gene Names: HCCA2
• UniProt Entry Name: MOB2_HUMAN

Uniprot Description

HCCA2: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: metal ion binding

Biological Process: positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis