Western Blot (WB)
(Western Blot analysis of MOB2 in Mouse liver tissue and Rat liver tissue lysates in order using MOB2 Rabbit pAb at dilution 1/400)
Rabbit MOB2 Polyclonal Antibody | anti-MOB2 antibody
MOB2 Rabbit pAb
Gene Names
MOB2; HCCA2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
MOB2; Polyclonal Antibody; MOB2 Rabbit pAb; MOB kinase activator 2; HCCA2; anti-MOB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.6mg/ml (varies by lot)
Applicable Applications for anti-MOB2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:20-1:100
IHC-P: 1:20-1:100
Immunogen
Synthetic peptide of human MOB2
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of MOB2 in Mouse liver tissue and Rat liver tissue lysates in order using MOB2 Rabbit pAb at dilution 1/400)
Western Blot (WB)
(Western Blot analysis of MOB2 in Mouse liver tissue and Rat liver tissue lysates in order using MOB2 Rabbit pAb at dilution 1/400)
Immunohistochemistry (IHC)
(Immunohistochemistry of MOB2 in paraffin-embedded Human liver cancer tissue using MOB2 Rabbit pAb at dilution 1/20. Predicted cell location: Cytoplasm)
Immunohistochemistry (IHC)
(Immunohistochemistry of MOB2 in paraffin-embedded Human liver cancer tissue using MOB2 Rabbit pAb at dilution 1/20. Predicted cell location: Cytoplasm)
Related Product Information for anti-MOB2 antibody
MOB2 (Mps one binder kinase activator-like 2), also known as HCCA2 (Hepatocellular carcinoma-associated gene 2), is a 467 amino acid protein that belongs to the MOB1/phocein family. MOB2 is expressed in lung, spleen, brain, and fetal liver. It is highly expressed in hepatocellular carcinoma. MOB2 is localized in the perinuclear region of the cytoplasm in liver cancer tissues and colocalizes with MAD2L2 in the nucleus of Hela cells. MOB2 is characterized by two N-glycosylation sites, six N-myristolylation sites, two Src homology 3 (SH3), and several phosphorylation motifs which indicate that this protein may play a role in an intracellular signal transduction cascade. MOB2 binds to and regulates the autophosphorylation of the related human serine/threonine kinase 38 (NDR1) and serine/threonine kinase 38L(NDR2). It has been shown that MOB2 plays a critical role in cell cycle regulation. Overexpression of the protein during the G0/G1 phase inhibits cell proliferation causing cell cycle arrest. Stimulates the autophosphorylation and kinase activity of STK38 and STK38L.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,174 Da
NCBI Official Full Name
MOB kinase activator 2 isoform 1
NCBI Official Synonym Full Names
MOB kinase activator 2
NCBI Official Symbol
MOB2
NCBI Official Synonym Symbols
HCCA2
NCBI Protein Information
MOB kinase activator 2; MOB2 Mps One Binder homolog; Mps one binder kinase activator-like 2
UniProt Protein Name
MOB kinase activator 2
Protein Family
UniProt Gene Name
MOB2
UniProt Synonym Gene Names
HCCA2
UniProt Entry Name
MOB2_HUMAN
Uniprot Description
HCCA2: Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: metal ion binding
Biological Process: positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis
Research Articles on MOB2
Similar Products
Product Notes
The MOB2 mob2 (Catalog #AAA4760943) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MOB2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MOB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:20-1:100. Researchers should empirically determine the suitability of the MOB2 mob2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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