Rabbit MKK5 Polyclonal Antibody | anti-MKK5 antibody

MKK5 (Phospho-S311) polyclonal antibody

Cat. Num. AAA3015064

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: MKK5; Polyclonal Antibody; MKK5 (Phospho-S311) polyclonal antibody; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MAP2K5; MEK5; PRKMK5; anti-MKK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MKK5 (Phospho-S311) polyclonal antibody detects endogenous levels of MKK5 protein only when phosphorylated at Ser311.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MKK5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human MKK5 around the phosphorylation site of Serine 311
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of MKK5 (Phospho-S311) polyclonal antibody at 1:500 dilutionLaneA: The Heart tissue lysate of MouseLaneB: The Heart tissue lysate of RatLaneC: H1792 whole cell lysateLaneD: MCF-7 whole cell lysate)

Related Product Information for anti-MKK5 antibody

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 332164798
• NCBI GeneID: 5607
• NCBI Accession #: NP_001193733.1
• NCBI GenBank Nucleotide #: NM_001206804.1
• UniProt Accession #: Q13163; Q92961; Q92962; B4DE43
• Molecular Weight: 50112 MW
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform C
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Uniprot Description

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.