Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat S-adenosylmethionine synthase isoform type-1 (MAT1A) Standard Curve.)
Rat S-Adenosylmethionine Synthase Isoform Type-1 (MAT1A) ELISA Kit | MAT1A elisa kit
Rat S-Adenosylmethionine Synthase Isoform Type-1 (MAT1A) ELISA Kit
Gene Names
Mat1a; SAS; SADE; AdoMet
Reactivity
Rat
Synonyms
S-Adenosylmethionine Synthase Isoform Type-1 (MAT1A); Rat S-Adenosylmethionine Synthase Isoform Type-1 (MAT1A) ELISA Kit; MAT1A; RP11-36D19.2; MAT; MATA1; SAMS; SAMS1; S-adenosylmethionine synthetase; MAT1A elisa kit
Reactivity
Rat
Specificity
Rat S-adenosylmethionine synthase isoform type-1 (MAT1A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAT1A. No significant cross-reactivity or interference between Rat MAT1A and analogues was observed.
Sequence Length
396
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat S-adenosylmethionine synthase isoform type-1 (MAT1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat S-adenosylmethionine synthase isoform type-1 (MAT1A) Standard Curve.)
Related Product Information for MAT1A elisa kit
Description: This Rat S-adenosylmethionine synthase isoform type-1 (MAT1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAT1A in samples. An antibody specific for MAT1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAT1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAT1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAT1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAT1a catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.The peptide maps of the 2 forms were possibly identical, although they showed different specific activities at physiologic concentrations of methionine. The findings suggested that the 2 isoforms are encoded by the same gene.
Background: MAT1a catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.The peptide maps of the 2 forms were possibly identical, although they showed different specific activities at physiologic concentrations of methionine. The findings suggested that the 2 isoforms are encoded by the same gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
S-adenosylmethionine synthase isoform type-1
NCBI Official Synonym Full Names
methionine adenosyltransferase 1A
NCBI Official Symbol
Mat1a
NCBI Official Synonym Symbols
SAS; SADE; AdoMet
NCBI Protein Information
S-adenosylmethionine synthase isoform type-1
UniProt Protein Name
S-adenosylmethionine synthase isoform type-1
Protein Family
UniProt Gene Name
Mat1a
UniProt Synonym Gene Names
Ams1; AdoMet synthase 1; MAT 1; MAT-I/III
NCBI Description
enzyme that catalyzes the formation of S-adenosylmethionine [RGD, Feb 2006]
Uniprot Description
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Research Articles on MAT1A
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Product Notes
The Rat MAT1A mat1a (Catalog #AAA9712322) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712322 ELISA Kit recognizes Rat MAT1A. It is sometimes possible for the material contained within the vial of "S-Adenosylmethionine Synthase Isoform Type-1 (MAT1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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