Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chordin-like protein 2 (CHRDL2) Standard Curve.)
Mouse Chordin-Like Protein 2 (CHRDL2) ELISA Kit | CHRDL2 elisa kit
Mouse Chordin-Like Protein 2 (CHRDL2) ELISA Kit
Gene Names
Chrdl2; Chl2; 1810022C01Rik
Reactivity
Mouse
Synonyms
Chordin-Like Protein 2 (CHRDL2); Mouse Chordin-Like Protein 2 (CHRDL2) ELISA Kit; CHRDL2; BNF1; CHL2; DKFZp586N2124; FKSG37; breast tumor novel factor 1; CHRDL2 elisa kit
Reactivity
Mouse
Specificity
Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse CHRDL2. No significant cross-reactivity or interference between Mouse CHRDL2 and analogues was observed.
Sequence Length
433
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chordin-like protein 2 (CHRDL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Chordin-like protein 2 (CHRDL2) Standard Curve.)
Related Product Information for CHRDL2 elisa kit
Description: This Mouse Chordin-like protein 2 (CHRDL2) ELISA Kit employs a two-site sandwich ELISA to quantitate CHRDL2 in samples. An antibody specific for CHRDL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRDL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRDL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRDL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 429-amino acid human protein shares 73% homology with the 426-amino acid mouse protein. CHL2 contains an N-terminal signal peptide, followed by 3 chordin (CHRD)-like cysteine-rich repeats (CR1 through CR3) and a C-terminal tail. Northern blot analysis detected high expression of a 2.0-kb CHL2 transcript in adult human uterus, with lower expression in colon, heart, prostate, stomach, and skeletal muscle. Expression was weak in placenta, testis, small intestine, trachea, and bone marrow, and no expression was detected in liver or kidney. In situ hybridization of mouse embryos showed that Chl2 expression was restricted to chondrocytes of developing joint cartilage. In adult mice, Chl2 expression was detected in connective tissues of reproductive organs, with highest expression in uterine myometrium.
Background: The deduced 429-amino acid human protein shares 73% homology with the 426-amino acid mouse protein. CHL2 contains an N-terminal signal peptide, followed by 3 chordin (CHRD)-like cysteine-rich repeats (CR1 through CR3) and a C-terminal tail. Northern blot analysis detected high expression of a 2.0-kb CHL2 transcript in adult human uterus, with lower expression in colon, heart, prostate, stomach, and skeletal muscle. Expression was weak in placenta, testis, small intestine, trachea, and bone marrow, and no expression was detected in liver or kidney. In situ hybridization of mouse embryos showed that Chl2 expression was restricted to chondrocytes of developing joint cartilage. In adult mice, Chl2 expression was detected in connective tissues of reproductive organs, with highest expression in uterine myometrium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
chordin-like protein 2 isoform 1
NCBI Official Synonym Full Names
chordin-like 2
NCBI Official Symbol
Chrdl2
NCBI Official Synonym Symbols
Chl2; 1810022C01Rik
NCBI Protein Information
chordin-like protein 2
UniProt Protein Name
Chordin-like protein 2
Protein Family
UniProt Gene Name
Chrdl2
UniProt Synonym Gene Names
Chl2
Uniprot Description
Implicated in tumor angiogenesis (). May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.
Research Articles on CHRDL2
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Product Notes
The Mouse CHRDL2 chrdl2 (Catalog #AAA9718104) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718104 ELISA Kit recognizes Mouse CHRDL2. It is sometimes possible for the material contained within the vial of "Chordin-Like Protein 2 (CHRDL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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