Rabbit JNK1 Monoclonal Antibody | anti-Mapk8 antibody

JNK1 Rabbit mAb

Cat. Num. AAA4752352

• Gene Names: Mapk8; JNK; JNK1; Prkm8; SAPK1; AI849689
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: JNK1; Monoclonal Antibody; JNK1 Rabbit mAb; JNK; Prkm8; SAPK1; AI849689; anti-Mapk8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R05-7A3
• Specificity: Brain (at protein level).
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-Mapk8 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: Recombinant protein of human JNK1
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of JNK1 in Jurkat,Rat Brain,C6 cell lysates using JNK1 Rabbit mAb(1:1000 diluted). Predicted band size:48kDa. Observed band size:46kDa.)

Related Product Information for anti-Mapk8 antibody

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (PubMed:29153991).

NCBI and Uniprot Product Information

• NCBI GI #: 7710060
• NCBI GeneID: 26419
• NCBI Accession #: NP_057909.1
• NCBI GenBank Nucleotide #: NM_016700.4
• UniProt Accession #: Q91Y86
• Molecular Weight: 44,229 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK; JNK1; Prkm8; SAPK1; AI849689
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• Protein Family: JNK1/MAPK8-associated membrane protein
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8
• UniProt Entry Name: MK08_MOUSE

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; mitochondrion; nucleus; perikaryon; podosome; vesicle

Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; MAP kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: dendrite morphogenesis; determination of dorsal identity; inflammatory response; JNK cascade; JUN phosphorylation; negative regulation of apoptosis; negative regulation of protein binding; neurite development; neuron migration; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of DNA replication; positive regulation of microtubule polymerization; positive regulation of neuron apoptosis; positive regulation of protein metabolic process; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of gene expression; regulation of histone deacetylation; regulation of protein localization; regulation of transcription, DNA-dependent; response to cadmium ion; response to heat; response to hydrogen peroxide; response to osmotic stress; response to UV; rhythmic process; signal transduction