Mouse Mitogen-activated protein kinase 8, MAPK8 ELISA Kit | MAPK8 elisa kit

Mouse Mitogen-activated protein kinase 8, MAPK8 ELISA Kit

Cat. Num. AAA2602636

• Gene Names: Mapk8; JNK; JNK1; Prkm8; SAPK1; AI849689
• Reactivity: Mouse
• Synonyms: Mitogen-activated protein kinase 8; MAPK8; Mouse Mitogen-activated protein kinase 8; MAPK8 ELISA Kit; MAPK8 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MAPK8 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPK8 elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse MAPK8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 7710060
• NCBI GeneID: 26419
• NCBI Accession #: NP_057909.1
• NCBI GenBank Nucleotide #: NM_016700.4
• UniProt Accession #: Q91Y86
• Molecular Weight: 44,229 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK; JNK1; Prkm8; SAPK1; AI849689
• NCBI Protein Information: mitogen-activated protein kinase 8; MAPK 8; MAP kinase 8; JNK1 beta1 protein kinase; c-Jun N-terminal kinase 1; mitogen activated protein kinase 8; protein kinase mitogen-activated 8; stress-activated protein kinase JNK1
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8
• UniProt Entry Name: MK08_MOUSE

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Cellular Component: dendrite cytoplasm; mitochondrion; axon; cytoplasm; perikaryon; nucleus; cytosol; vesicle

Molecular Function: MAP kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; JUN kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of microtubule polymerization; rhythmic process; signal transduction; protein amino acid phosphorylation; regulation of protein localization; regulation of transcription, DNA-dependent; JNK cascade; inflammatory response; negative regulation of protein binding; neurite development; response to UV; regulation of histone deacetylation; ossification; peptidyl-threonine phosphorylation; determination of dorsal identity; response to osmotic stress; regulation of circadian rhythm; JUN phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; response to hydrogen peroxide; response to heat; regulation of gene expression; phosphorylation; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration