Rabbit anti-Human Mapk8 Polyclonal Antibody | anti-Mapk8 antibody

Mapk8, ID (Mapk8, Jnk1, Prkm8, Mitogen-activated protein kinase 8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1) (Biotin)

Cat. Num. AAA6318860

• Gene Names: Mapk8; JNK; JNK1; Prkm8; SAPK1; AI849689
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Mapk8; Polyclonal Antibody; ID (Mapk8; Jnk1; Prkm8; Mitogen-activated protein kinase 8; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1) (Biotin); c-Jun N-terminal kinase 1; anti-Mapk8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Mapk8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Mapk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-268 amino acids from the Central region of Mouse Mapk8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Mapk8 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Mapk8 antibody detected the Mapk8 protein (arrow).)

Related Product Information for anti-Mapk8 antibody

Mapk8 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Phosphorylates heat shock factor protein 4 (HSF4) (By similarity).

Product Categories/Family for anti-Mapk8 antibody

Antibodies; Protein Kinases

References

Sherrin, T., et al. J. Neurosci. 30(40):13348-13361(2010). Morel, C., et al. Mol. Cell. Biol. 30(19):4616-4625(2010). Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010). Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010). Cellurale, C., et al. PLoS ONE 5 (8), E12469 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7710060
• NCBI GeneID: 26419
• NCBI Accession #: NP_057909.1
• NCBI GenBank Nucleotide #: NM_016700.4
• UniProt Accession #: Q91Y86
• Molecular Weight: 44,229 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK; JNK1; Prkm8; SAPK1; AI849689
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8
• UniProt Entry Name: MK08_MOUSE

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; mitochondrion; nucleus; perikaryon; podosome; vesicle

Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; MAP kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: dendrite morphogenesis; determination of dorsal identity; inflammatory response; JNK cascade; JUN phosphorylation; negative regulation of apoptosis; negative regulation of protein binding; neurite development; neuron migration; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of DNA replication; positive regulation of microtubule polymerization; positive regulation of neuron apoptosis; positive regulation of protein metabolic process; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of gene expression; regulation of histone deacetylation; regulation of protein localization; regulation of transcription, DNA-dependent; response to cadmium ion; response to heat; response to hydrogen peroxide; response to osmotic stress; response to UV; rhythmic process; signal transduction