Mitogen Activated Protein Kinase 8 (MAPK8) Recombinant Protein | MAPK8 recombinant protein

Recombinant Mitogen Activated Protein Kinase 8 (MAPK8)

Cat. Num. AAA2010164

• Gene Names: Mapk8; JNK
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mitogen Activated Protein Kinase 8 (MAPK8); Recombinant Mitogen Activated Protein Kinase 8 (MAPK8); MAPK8 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC LKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG
• Sequence Length: 427

• Applicable Applications for MAPK8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly138~Gly370 (Accession # P49185) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 395627627
• NCBI GeneID: 116554
• NCBI Accession #: NP_446281.1
• NCBI GenBank Nucleotide #: NM_053829.1
• UniProt Accession #: P49185
• Molecular Weight: 30.4kDa
• NCBI Official Full Name: mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK
• NCBI Protein Information: mitogen-activated protein kinase 8; MAPK 8; p54 gamma; SAPK gamma; MAP kinase 8; c-Jun N-terminal kinase 1; c-jun NH2-terminal kinase; stress-activated protein kinase JNK1
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8
• UniProt Entry Name: MK08_RAT

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Cellular Component: dendrite cytoplasm; mitochondrion; axon; cytoplasm; perikaryon; cytosol; nucleus; vesicle

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; histone deacetylase regulator activity; histone deacetylase binding; kinase activity; JUN kinase activity; ATP binding

Biological Process: positive regulation of apoptosis; positive regulation of microtubule polymerization; rhythmic process; signal transduction; protein amino acid phosphorylation; regulation of protein localization; regulation of transcription, DNA-dependent; JNK cascade; negative regulation of protein binding; inflammatory response; neurite development; response to UV; regulation of histone deacetylation; ossification; peptidyl-threonine phosphorylation; response to osmotic stress; regulation of circadian rhythm; JUN phosphorylation; peptidyl-serine phosphorylation; response to cadmium ion; response to hydrogen peroxide; regulation of gene expression; response to steroid hormone stimulus; response to heat; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cell migration