Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Discs Polyclonal Antibody | anti-DLG5 antibody
Polyclonal Antibody to Discs, Large Homolog 5 (DLG5)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type PC3 cell lysate; Lane 2: DLG5 knockout PC3 cell lysate; Predicted MW: 214kd Observed MW: 200kd Primary Ab: 2ug/ml Rabbit Anti-Mouse DLG5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
DLG5: May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton. Belongs to the MAGUK family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 14|14 A3
Cellular Component: cell junction; cell projection; cell-cell adherens junction; cytoplasm; cytoskeleton; membrane; plasma membrane; postsynaptic density; postsynaptic membrane; synapse
Molecular Function: beta-catenin binding; cytoskeletal protein binding; protein binding
Biological Process: apical protein localization; epithelial to mesenchymal transition; establishment or maintenance of epithelial cell apical/basal polarity; maintenance of cell polarity; midbrain development; negative regulation of cell migration; negative regulation of T cell proliferation; polarized epithelial cell differentiation; positive regulation of smoothened signaling pathway; positive regulation of synaptogenesis; protein complex assembly; regulation of apoptosis; zonula adherens assembly
Research Articles on DLG5
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Product Notes
The DLG5 dlg5 (Catalog #AAA2033859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Discs, Large Homolog 5 (DLG5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Discs can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the DLG5 dlg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Discs, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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