HPGD recombinant protein

HPGD, 1-266aa, Human, His tag, E Coli

Cat. Num. AAA203705

• Gene Names: HPGD; PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HPGD; 1-266aa; Human; His tag; E Coli; 15-hydroxyprostaglandin dehydrogenase [NAD+]; 15-PGDH; PGDH; PGDH1; SDR36C1; Prostaglandin dehydrogenase 1; HPGD recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG IHFQDYDTTP FQAKTQ
• Sequence Length: 266

• Applicable Applications for HPGD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HPGD recombinant protein

HPGD, also known as 15-PGDH, is the fundamental enzyme of prostaglandin degradation. This protein strongly reduces the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. HPGD is implicated in many physiologic and cellular processes, such as inflammation. Recombinant human HPGD protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for HPGD recombinant protein

Immunology

References

Han X., et al. (1995) Equine Vet. J. 27: 334-339.; Van Meir C.A., et al. (1996) Placenta 17: 291-297.;

NCBI and Uniprot Product Information

• NCBI GI #: 31542939
• NCBI GeneID: 3248
• NCBI Accession #: NP_000851
• NCBI GenBank Nucleotide #: NM_000860.5
• UniProt Accession #: P15428; O00749; Q06F08; Q12998; B4DTA4; B4DU74; B4DV57; D3DP43; E7EV11
• Molecular Weight: 31.1 (286aa), confirmed by MALDI-TOF
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Symbol: HPGD
• NCBI Official Synonym Symbols: PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• Protein Family: 15-hydroxyprostaglandin dehydrogenase
• UniProt Gene Name: HPGD
• UniProt Synonym Gene Names: PGDH1; SDR36C1; 15-PGDH

NCBI Description

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.