MIP-1 alpha/CCL3 Recombinant Protein | CCL3 recombinant protein

MIP-1 alpha/CCL3 Protein, Rat, Recombinant

Cat. Num. AAA8123341

• Gene Names: Ccl3; Scya3; MIP-1a
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MIP-1 alpha/CCL3; MIP-1 alpha/CCL3 Protein; Rat; Recombinant; Rat CCL3/Mip1a Protein; chemokine (C-C motif) ligand 3; CCL3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala24-Ala92

• Species: Rat
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the rat CCL3 (P50229)(Ala24-Ala92) was expressed and purified with an initial Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL3 recombinant protein

Background: CCL3 is a cytokine belonging to the CC chemokine family. Chemokines are a family of structurally related leukocyte chemoattractant cytokines that play a central role during immunoregulatory and inflammation processes. All chemokines contain four conserved cysteines linked by disulfide bonds, and two major subfamilies, namely CXC and CC, are defined on the basis of the first two cysteines which are separated by one amino acid or are adjacent. CCL3 is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes.

Product Categories/Family for CCL3 recombinant protein

Neuroinflammation

References

Zhao RY, et al. (2005) Viral infections and cell cycle G2/M regulation. Cell Res. 15(3):143-9. Joseph AM, et al. (2005) Nef: "necessary and enforcing factor" in HIV infection. Curr HIV Res. 3(1):87-94. Muthumani K, et al. (2004) HIV-1 Vpr and anti-inflammatory activity. DNA Cell Biol. 23(4): 239-47.

NCBI and Uniprot Product Information

• NCBI GI #: 40254794
• NCBI GeneID: 25542
• NCBI Accession #: NP_037157.2
• NCBI GenBank Nucleotide #: NM_013025.2
• UniProt Accession #: P50229; A1EC87
• Molecular Weight: 10,335 Da
• NCBI Official Full Name: C-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 3
• NCBI Official Symbol: Ccl3
• NCBI Official Synonym Symbols: Scya3; MIP-1a
• NCBI Protein Information: C-C motif chemokine 3; MIP-1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; small inducible cytokine A3; small-inducible cytokine A3
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl3
• UniProt Synonym Gene Names: Mip1a; Scya3; MIP-1-alpha
• UniProt Entry Name: CCL3_RAT

NCBI Description

mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]

Uniprot Description

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.