Human Runt Related Transcription Factor 2 (RUNX2) RTU ELISA Kit | RUNX2 rtu elisa kit

Human Runt Related Transcription Factor 2 (RUNX2) ELISA Kit

Cat. Num. AAA4502296

• Gene Names: RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
• Reactivity: Human
• Synonyms: Runt Related Transcription Factor 2 (RUNX2); Human Runt Related Transcription Factor 2 (RUNX2) ELISA Kit; AML3; CBFA1; CCD1; OSF2; PEBP2A; Acute myeloid leukemia 3; Core-binding factor alpha-1; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 A; RUNX2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 499

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RUNX2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RUNX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RUNX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RUNX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RUNX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226442791
• NCBI GeneID: 860
• NCBI Accession #: NP_001015051.3
• NCBI GenBank Nucleotide #: NM_001015051.3
• UniProt Accession #: Q13950; O14614; O14615; O95181
• Molecular Weight: 54,249 Da
• NCBI Official Full Name: runt-related transcription factor 2 isoform b
• NCBI Official Synonym Full Names: runt related transcription factor 2
• NCBI Official Symbol: RUNX2
• NCBI Official Synonym Symbols: CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
• NCBI Protein Information: runt-related transcription factor 2
• UniProt Protein Name: Runt-related transcription factor 2
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: RUNX2
• UniProt Synonym Gene Names: AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A

NCBI Description

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Uniprot Description

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed:28505335, PubMed:28738062, PubMed:28703881). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (). Inhibits KAT6B-dependent transcriptional activation.