Rabbit anti-Human MFN1 Polyclonal Antibody | anti-MFN1 antibody

MFN1, ID (MFN1, Mitofusin-1, Fzo homolog, Transmembrane GTPase MFN1) (FITC)

Cat. Num. AAA6320528

• Gene Names: MFN1; hfzo1; hfzo2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MFN1; Polyclonal Antibody; ID (MFN1; Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1) (FITC); Transmembrane GTPase MFN1; anti-MFN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MFN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MFN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the Central region of human MFN1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MFN1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MFN1 antibody detected the MFN1 protein (arrow).)

Related Product Information for anti-MFN1 antibody

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.

Product Categories/Family for anti-MFN1 antibody

Antibodies; Transmembrane Proteins

References

Perumalsamy, L.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6882-6887(2010). Park, Y.Y., et al. J. Cell. Sci. 123 (PT 4), 619-626 (2010): Onoguchi, K., et al. PLoS Pathog. 6 (7), E1001012 (2010): Waxman, A.B., et al. Am. J. Respir. Cell Mol. Biol. 41(4):385-396(2009). Wolf, C., et al. BMC Med. Genet. 10, 91 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 45269137
• NCBI GeneID: 55669
• NCBI Accession #: NP_284941.2
• NCBI GenBank Nucleotide #: NM_033540.2
• UniProt Accession #: Q8IWA4; O15323; O60639; Q9BZB5; Q9NWQ2; B2RAR1; D3DNR6
• Molecular Weight: 71,401 Da
• NCBI Official Full Name: mitofusin-1
• NCBI Official Synonym Full Names: mitofusin 1
• NCBI Official Symbol: MFN1
• NCBI Official Synonym Symbols: hfzo1; hfzo2
• NCBI Protein Information: mitofusin-1
• UniProt Protein Name: Mitofusin-1
• Protein Family: Mitofusin
• UniProt Gene Name: MFN1
• UniProt Entry Name: MFN1_HUMAN

NCBI Description

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

Uniprot Description

MFN1: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. Belongs to the mitofusin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; EC 3.6.5.-

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: mitochondrial outer membrane; integral to membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: mitochondrial fusion; metabolic process