Rabbit Methylated Lysine Polyclonal Antibody

Methylated Lysine Antibody: HRP

Cat. Num. AAA805807

• Reactivity: Multi species
• Applications: Western Blot, ELISA
• Synonyms: Methylated Lysine; Polyclonal Antibody; Methylated Lysine Antibody: HRP; dimethylysine; methyl lysine; N epsilon dimethyl lysine; trimethyl lysine; anti-Methylated Lysine antibody

• Host: Rabbit
• Reactivity: Multi species
• Clonality: Polyclonal
• Form/Format: Affinity Purified, HRP conjugated
• Concentration: 250ug/mL (varies by lot)

• Applicable Applications for anti-Methylated Lysine antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: 1:2000-1:5000 dilution WB
• Certificate of Analysis: 0.2-0.5 ug/mL of SPC-160 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
• Conjugate: HRP
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).)

Related Product Information for anti-Methylated Lysine antibody

Background Info: Detects proteins containing methylated lysine residues in SDS PAGE immunoblots.

Scientific Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Product Categories/Family for anti-Methylated Lysine antibody

Cell Signaling; Post-translational Modifications

References

1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Melcher M. et al. (2000). Mol Cell Biol 20: 3728- 3741. 3. Bannister AJ. et al. (2001). Nature 410(6824): 120-4. 4. Im H. et al. (2003). J Biol Chem. 278: 18346-18352. 5. Ng H.H., et al. (2002). J Biol Chem. 277: 34655-34657. 6. Zegerman P., et al. (2002). J Biol Chem. 277: 11621- 11624.