Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).)
Rabbit Methylated Lysine Polyclonal Antibody
Methylated Lysine Antibody: RPE
Reactivity
Species Independent
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Purity
Protein A Purified
Synonyms
Methylated Lysine; Polyclonal Antibody; Methylated Lysine Antibody: RPE; Rabbit Anti-Methylated Lysine Polyclonal; Dimethylysine Antibody; Methyl lysine Antibody; N epsilon dimethyl lysine Antibody; Trimethyl lysine Antibody; anti-Methylated Lysine antibody
Host
Rabbit
Reactivity
Species Independent
Clonality
Polyclonal
Specificity
Detects proteins containing methylated lysine residues.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Methylated Lysine antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
WB: 1:20000
ICC/IF: 1:200
ICC/IF: 1:200
Target
Methylated Lysine
Immunogen
Methylated KLH Conjugated
Conjugate
RPE
Storage Buffer
PBS, 50% glycerol
PubMed ID
29056417; 27858264; 27521690; 25609799; 25374779; 24651940; 24233354; 23533717
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).)
Western Blot (WB)
(Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).)
Related Product Information for anti-Methylated Lysine antibody
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
Product Categories/Family for anti-Methylated Lysine antibody
References
1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
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Product Notes
The Methylated Lysine (Catalog #AAA809380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Methylated Lysine Antibody: RPE reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's Methylated Lysine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA). WB: 1:20000 ICC/IF: 1:200. Researchers should empirically determine the suitability of the Methylated Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methylated Lysine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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