Rabbit anti-Mouse Methionyl tRNA Synthetase Polyclonal Antibody | anti-MARS antibody

Polyclonal Antibody to Methionyl tRNA Synthetase (MARS)

Cat. Num. AAA2034027

• Gene Names: Mars; Metrs; Mtrns
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Methionyl tRNA Synthetase; Polyclonal Antibody; Polyclonal Antibody to Methionyl tRNA Synthetase (MARS); METRS; MTRNS; MetRS; Methionine tRNA Ligase 1; Cytoplasmic; anti-MARS antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MARS. It has been selected for its ability to recognize MARS in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 915

• Applicable Applications for anti-MARS antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-1:5000
• Fragment: MARS (Gly74~Pro212)
• Conjugate: No Conjugate
• Cross Reactivity: Human, Mouse
• Immunoglobulin Type: IgG
• Quality Control: Content: The quality control contains recombinant MARS (Gly74~Pro212) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2069428
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse MARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

Knockout Validation

(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: MARS knockout HepG2 cell lysate; Predicted MW: 101kd Observed MW: 90kd Primary Ab: 1ug/ml Rabbit Anti-Mouse MARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Product Categories/Family for anti-MARS antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 51491852
• NCBI GeneID: 216443
• NCBI Accession #: NP_001003913.1
• NCBI GenBank Nucleotide #: NM_001003913.2
• UniProt Accession #: Q68FL6
• Molecular Weight: 101,431 Da
• NCBI Official Full Name: methionine--tRNA ligase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: methionine-tRNA synthetase
• NCBI Official Symbol: Mars
• NCBI Official Synonym Symbols: Metrs; Mtrns
• NCBI Protein Information: methionine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Methionine--tRNA ligase, cytoplasmic
• Protein Family: Methionine--tRNA ligase
• UniProt Gene Name: Mars
• UniProt Synonym Gene Names: MetRS

NCBI Description

The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010]

Uniprot Description

MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]

Protein type: Aminoacyl-tRNA synthetase; EC 6.1.1.10; Ligase; Other Amino Acids Metabolism - selenoamino acid; Translation; Translation regulation

Chromosomal Location of Human Ortholog: 10|10 D3

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; membrane; mitochondrion; nucleolus

Molecular Function: aminoacyl-tRNA ligase activity; ATP binding; ligase activity; methionine-tRNA ligase activity; nucleotide binding; RNA binding; tRNA binding

Biological Process: cellular response to insulin stimulus; cellular response to starvation; methionyl-tRNA aminoacylation; rRNA transcription; translation; tRNA aminoacylation for protein translation