Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse TST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Thiosulfate Sulfurtransferase Polyclonal Antibody | anti-TST antibody
Thiosulfate Sulfurtransferase (TST) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse TST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse TST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: TST knockout HepG2 cell lysate; Predicted MW: 33kd Observed MW: 33kd Primary Ab: 1ug/ml Rabbit Anti-Mouse TST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: TST knockout HepG2 cell lysate; Predicted MW: 33kd Observed MW: 33kd Primary Ab: 1ug/ml Rabbit Anti-Mouse TST Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Protein type: EC 2.8.1.1; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 15 E1|15 37.47 cM
Cellular Component: extracellular space; mitochondrial inner membrane; mitochondrion
Molecular Function: 5S rRNA binding; RNA binding; thiosulfate sulfurtransferase activity; transferase activity
Biological Process: epithelial cell differentiation; rRNA transport
Research Articles on TST
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Product Notes
The TST tst (Catalog #AAA2032394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thiosulfate Sulfurtransferase (TST) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Thiosulfate Sulfurtransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TST tst for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thiosulfate Sulfurtransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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