Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Aspartyl tRNA Synthetase Polyclonal Antibody | anti-DARS antibody
Polyclonal Antibody to Aspartyl tRNA Synthetase (DARS)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human DARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DARS knockout HepG2 cell lysate; Predicted MW: 46,57kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human DARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: DARS knockout HepG2 cell lysate; Predicted MW: 46,57kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human DARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.
Protein type: Aminoacyl-tRNA synthetase; EC 6.1.1.12; Ligase; Translation; Translation regulation
Chromosomal Location of Human Ortholog: 2q21.3
Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; membrane
Molecular Function: aminoacylase activity; aspartate-tRNA ligase activity; ATP binding; protein binding; RNA binding
Biological Process: aspartyl-tRNA aminoacylation; protein complex assembly; translation; tRNA aminoacylation for protein translation
Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity
Research Articles on DARS
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Product Notes
The DARS dars (Catalog #AAA2033853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aspartyl tRNA Synthetase (DARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the DARS dars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartyl tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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