Sheep c-fos ELISA Kit | c-fos elisa kit

Sheep c-fos ELISA Kit

Cat. Num. AAA2512516

• Gene Names: FOS; p55; AP-1; C-FOS
• Reactivity: Sheep
• Synonyms: c-fos; Sheep c-fos ELISA Kit; c-fos elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep c-fos. No significant cross-reactivity or interference between Sheep c-fos and analogues was observed.
• Sequence Length: 380

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL ; Max: 5000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep c-fos concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for c-fos elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to c-fos. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for c-fos and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain c-fos, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of c-fos. You can calculate the concentration of c-fos in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 29904
• NCBI GeneID: 2353
• UniProt Accession #: P01100; P18849; A8K4E2; B4DQ65
• Molecular Weight: 36,301 Da
• NCBI Official Full Name: c-fos
• NCBI Official Synonym Full Names: FBJ murine osteosarcoma viral oncogene homolog
• NCBI Official Symbol: FOS
• NCBI Official Synonym Symbols: p55; AP-1; C-FOS
• NCBI Protein Information: proto-oncogene c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); G0/G1 switch regulatory protein 7; activator protein 1; cellular oncogene c-fos
• UniProt Protein Name: Proto-oncogene c-Fos
• UniProt Gene Name: FOS
• UniProt Synonym Gene Names: G0S7
• UniProt Entry Name: FOS_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytosol; nucleus

Molecular Function: protein binding; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; response to toxin; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; conditioned taste aversion; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; aging; response to corticosterone stimulus; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; toll-like receptor 2 signaling pathway; cellular response to hormone stimulus; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus