Western Blot (WB)
(Western blot analysis of mIMCD-3 cell extracts stimulated with 40 ng/ml HGF for 2 minutes, using Phospho-Met (Tyr1349) Antibody, MBS623904 (upper) and control Met antibody (lower).)
Rabbit Met Polyclonal Antibody | anti-met antibody
Met, phosphorylated (Tyr1349)
Gene Names
met; c-met; met-A
Reactivity
Human, Monkey, Mouse
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by protein A and immunoaffinity chromatography.
Synonyms
Met; Polyclonal Antibody; phosphorylated (Tyr1349); Anti -Met; anti-met antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Met only when phosphorylated at Tyr1349. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by protein A and immunoaffinity chromatography.
Purified by protein A and immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Applicable Applications for anti-met antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunogen
Synthetic phosphotyrosine corresponding aa residues surrounding Tyrosine 1349 of human Met (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of mIMCD-3 cell extracts stimulated with 40 ng/ml HGF for 2 minutes, using Phospho-Met (Tyr1349) Antibody, MBS623904 (upper) and control Met antibody (lower).)
Western Blot (WB)
(Western blot analysis of mIMCD-3 cell extracts stimulated with 40 ng/ml HGF for 2 minutes, using Phospho-Met (Tyr1349) Antibody, MBS623904 (upper) and control Met antibody (lower).)
Western Blot (WB)
(Western blot analysis of Vero cell extracts treated with 3 nM In1B, using Phospho-Met (Tyr1349) Antibody (upper) and control Met antibody (lower). In1B is a c-Met activator (Shen, Y. et al. 2000, Cell 103, 501-510). (In1B provided by Dr. K. Ireton, University of Toronto, Canada.))
Western Blot (WB)
(Western blot analysis of Vero cell extracts treated with 3 nM In1B, using Phospho-Met (Tyr1349) Antibody (upper) and control Met antibody (lower). In1B is a c-Met activator (Shen, Y. et al. 2000, Cell 103, 501-510). (In1B provided by Dr. K. Ireton, University of Toronto, Canada.))
Related Product Information for anti-met antibody
Met is a high affinity receptor for hepatocyte
Product Categories/Family for anti-met antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
met proto-oncogene
NCBI Official Synonym Full Names
met proto-oncogene
NCBI Official Symbol
met
NCBI Official Synonym Symbols
c-met; met-A
NCBI Protein Information
met proto-oncogene; c-met/hepatocyte growth factor receptor; met proto-oncogene (hepatocyte growth factor receptor)
Protein Family
Research Articles on met
Similar Products
Product Notes
The met (Catalog #AAA623904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Met, phosphorylated (Tyr1349) reacts with Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Met can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Met, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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