Rabbit anti-Human Ephrin B Polyclonal Antibody

Ephrin B, phosphorylated (Tyr324/329)

Cat. Num. AAA623735

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Ephrin B; Polyclonal Antibody; phosphorylated (Tyr324/329); Anti -Ephrin B; anti-Ephrin B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes transfected levels of human Ephrin B at ~59kD only when phosphorylated at Tyr324/329. Cossreacts with ephrins B1, B2 and B3. Does not crossreact with other tyrosine-phosphorylated proteins.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-Ephrin B antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphotyrosine peptide corresponding to residues surrounding Tyr324/329 of human ephrin B1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of cell lysates from NIH/3T3 stably expressing ephrin B1 stimulated with its cognate receptor EphB2-Fc or unfused Fc (6), using MBS623735 (upper) and control ephrin B antibody (lower).)

Related Product Information for anti-Ephrin B antibody

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, and EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases and cancers (3). Both ephrin A and ephrin B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling" whereby their cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as a major tyrosine phosphorylation sites of ephrin B1 in vivo (7).

Product Categories/Family for anti-Ephrin B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 31793130
• NCBI Accession #: NP_855623.1
• NCBI GenBank Nucleotide #: NC_002945.3
• NCBI Official Full Name: epoxide hydrolase EphB