Western Blot (WB)
(Western Blot (WB) analysis of Mena (N504) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (20ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:The Prostate tissue lysate of Rat (40ug) Lane4:The Embryo tissue lysate of Mouse (40ug))
Rabbit Mena Polyclonal Antibody | anti-Mena antibody
Mena (N504) polyclonal antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Mena ; Polyclonal Antibody; Mena (N504) polyclonal antibody; Protein enabled homolog; ENAH; MENA; anti-Mena antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Mena (N504) polyclonal antibody detects endogenous levels of Mena protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-Mena antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 474-534 of Human Mena.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Mena (N504) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (20ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:The Prostate tissue lysate of Rat (40ug) Lane4:The Embryo tissue lysate of Mouse (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Mena (N504) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (20ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:The Prostate tissue lysate of Rat (40ug) Lane4:The Embryo tissue lysate of Mouse (40ug))
Related Product Information for anti-Mena antibody
Mena (mammalian enabled), EVL, and VASP are members of the Ena/VASP family, which is involved in controlling cell shape and cell movement by shielding actin filaments from capping proteins. Ena/VASP proteins have three specific domains: an amino-terminal EVH1 domain controlling protein localization; a central proline-rich domain mediating interactions with both SH3 and WW domain containing proteins, including profilin; and a carboxy-terminal domain causing tetramerization and binding to actin. Mena interacts with actin filaments at the growing ends localizing to lamellipodia and to tips of growth cone filopodia in neurons. Axons projecting from interhemispheric cortico-cortical neurons are misrouted in newborn, homozygous Mena knock-out mice. Mena is phosphorylated at Ser236 by PKA, thereby promoting filopodial formation and elongation in the growth cone. Three forms of Mena corresponding to 80, 88 and 140 kD are known. The 80 kD protein is broadly expressed in contrast to the 140 kD protein which is enriched in neural cell types. Alternative splicing produces these forms. The 88 kD protein is mainly found in embryonic cell types and is likely the result of post-translational modification. Expression of all three forms is completely eliminated in Mena homozygous mutant animals.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 88, 130kDa
Similar Products
Product Notes
The Mena (Catalog #AAA3014020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mena (N504) polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mena can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Mena for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mena , Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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