Rabbit SSH3 Polyclonal Antibody | anti-SSH3 antibody

SSH3 Polyclonal Antibody

Cat. Num. AAA3007550

• Gene Names: SSH3; SSH3L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SSH3; Polyclonal Antibody; SSH3 Polyclonal Antibody; Protein phosphatase Slingshot homolog 3; SSH-like protein 3; SSH-3L; hSSH-3L; SSH3L; anti-SSH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SSH3 polyclonal antibody detects endogenous levels of SSH3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 659

• Applicable Applications for anti-SSH3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human SSH3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SSH3 pAb at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-SSH3 antibody

Slingshot homolog 3 (SSH3) is a protein phosphatase that modifies actin cytoskeleton dynamics via cofilin dephosphorylation. Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms. The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3. The slingshot homologs (SSH1, SSH2, and, to a lesser extent, SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization. SSH3 is widely expressed in epithelial tissues, and has been found to be non-essential for viability and fertility in knockout mice. While its biological function remains elusive, phosphorylation at Ser37 of SSH3 has been identified in several phosphoproteomic studies.

NCBI and Uniprot Product Information

• NCBI GI #: 239582767
• NCBI GeneID: 54961
• NCBI Accession #: NP_060327.3
• NCBI GenBank Nucleotide #: NP_060327.3
• UniProt Accession #: Q8TE77
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: protein phosphatase Slingshot homolog 3
• NCBI Official Synonym Full Names: slingshot protein phosphatase 3
• NCBI Official Symbol: SSH3
• NCBI Official Synonym Symbols: SSH3L
• NCBI Protein Information: protein phosphatase Slingshot homolog 3
• UniProt Protein Name: Protein phosphatase Slingshot homolog 3
• Protein Family: Protein phosphatase Slingshot
• UniProt Gene Name: SSH3
• UniProt Synonym Gene Names: SSH3L; SSH-3L; hSSH-3L
• UniProt Entry Name: SSH3_HUMAN

NCBI Description

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM, Mar 2008]

Uniprot Description

SSH3: a protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Does not bind to, or colocalize with, filamentous actin. Five alternatively spliced isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; Protein phosphatase, Ser/Thr (non-receptor); Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.48; Cytoskeletal; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytoskeleton; cytoplasm; nucleus

Molecular Function: DNA binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; actin binding

Biological Process: regulation of actin polymerization and/or depolymerization; regulation of axonogenesis; protein amino acid dephosphorylation