Rabbit Caldesmon Polyclonal Antibody | anti-S783 antibody

Caldesmon (S783) Polyclonal Antibody

Cat. Num. AAA3001073

• Gene Names: CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Caldesmon; Polyclonal Antibody; Caldesmon (S783) Polyclonal Antibody; CDM; CALD1; CAD; anti-S783 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caldesmon (S783) polyclonal antibody detects endogenous levels of Caldesmon protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 538

• Applicable Applications for anti-S783 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 750-800 of Human Caldesmon.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Caldesmon (S783) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (20ug) Lane2:Hela whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Caldesmon (S783) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of Caldesmon (S783) pAb at 1:500 dilutionLane1:786-O whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug) Lane5:The heart tissue lysate of Mouse (40ug) Lane6:The heart tissue lysate of Rat (40ug))

Related Product Information for anti-S783 antibody

Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4826657
• NCBI GeneID: 800
• NCBI Accession #: NP_004333.1
• NCBI GenBank Nucleotide #: NP_004333.1
• UniProt Accession #: Q05682
• Molecular Weight: 70-80kDa non-muscle; 120-150kDa smooth muscle
• NCBI Official Full Name: caldesmon isoform 2
• NCBI Official Synonym Full Names: caldesmon 1
• NCBI Official Symbol: CALD1
• NCBI Official Synonym Symbols: CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
• NCBI Protein Information: caldesmon
• UniProt Protein Name: Caldesmon
• Protein Family: Caldesmon
• UniProt Gene Name: CALD1
• UniProt Synonym Gene Names: CAD; CDM; CDM
• UniProt Entry Name: CALD1_HUMAN

NCBI Description

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Caldesmon: a cytoskeletal protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells. May act as a bridge between myosin and actin filaments. Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Five alternatively spliced isoforms have been described.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoskeleton; myofibril; plasma membrane; actin cap; cytosol; actin cytoskeleton

Molecular Function: calmodulin binding; myosin binding; tropomyosin binding; actin binding

Biological Process: muscle contraction; cell motility