Rabbit anti-Human Daxx Polyclonal Antibody | anti-Daxx antibody

Daxx (Phospho-S668) Polyclonal Antibody

Cat. Num. AAA3004877

• Gene Names: DAXX; DAP6; EAP1; BING2; SMIM40
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Daxx; Polyclonal Antibody; Daxx (Phospho-S668) Polyclonal Antibody; Death domain-associated protein 6; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein; BING2; DAP6; DAXX; anti-Daxx antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Daxx (phospho-S668) polyclonal antibodydetects endogenous levels of Daxx protein only when phosphorylated at Ser668.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 740

• Applicable Applications for anti-Daxx antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Daxx around the phosphorylation site of Serine 668.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Daxx (S668) pAb at 1:500 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:K562 whole cell lysate (20ug))

Testing Data

Related Product Information for anti-Daxx antibody

Apoptosis, or programmed cell death, occurs duringnormal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family. A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx is a death domain containing important intermediate in the Fas mediated apoptosis. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway. It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 215422388
• NCBI GeneID: 1616
• NCBI Accession #: NP_001135441.1
• NCBI GenBank Nucleotide #: NP_001135441.1
• UniProt Accession #: Q9UER7
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2; SMIM40
• NCBI Protein Information: death domain-associated protein 6
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Transcription, coactivator/corepressor; Nucleolus; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; heat shock protein binding; ubiquitin protein ligase binding; receptor signaling protein activity; protein kinase activator activity; transcription corepressor activity

Biological Process: viral reproduction; transcription, DNA-dependent; regulation of protein ubiquitination; apoptosis; activation of JNK activity; chromatin remodeling; nucleosome assembly; cytokinesis after mitosis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent