Rabbit anti-Human, Mouse p130 Cas Polyclonal Antibody | anti-Cas antibody

p130 Cas (V404) Polyclonal Antibody

Cat. Num. AAA3001033

• Gene Names: BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: p130 Cas; Polyclonal Antibody; p130 Cas (V404) Polyclonal Antibody; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; BCAR1; CAS; CASS1; CRKAS; anti-Cas antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p130 Cas (V404) polyclonal antibody detects endogenous levels of p130 Cas protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 916

• Applicable Applications for anti-Cas antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 380-430 of Human p130 Cas.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BCAR1 pAb at 1:500 dilutionLane1:A549 whole cell lysate (20ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p130 Cas (V404) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of p130 Cas (V404) pAb at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Related Product Information for anti-Cas antibody

The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.

NCBI and Uniprot Product Information

• NCBI GI #: 282398112
• NCBI GeneID: 9564
• NCBI Accession #: NP_001164185.1
• NCBI GenBank Nucleotide #: NP_001164185.1
• UniProt Accession #: P56945
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 1 isoform 1
• NCBI Official Synonym Full Names: BCAR1 scaffold protein, Cas family member
• NCBI Official Symbol: BCAR1
• NCBI Official Synonym Symbols: CAS; CAS1; CASS1; CRKAS; P130Cas
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 1
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 1
• Protein Family: Exportin
• UniProt Gene Name: BCAR1
• UniProt Synonym Gene Names: CAS; CASS1; CRKAS
• UniProt Entry Name: BCAR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]

Uniprot Description

Function: Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. Ref.10 Ref.11

Subunit structure: Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.16

Subcellular location: Cell junction › focal adhesion. Cytoplasm. Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation

By similarity. Ref.10

Tissue specificity: Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Domain: Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9

By similarity.A serine-rich region promotes activation of the serum response element (SRE).The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

Post-translational modification: PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Ref.10 Ref.13 Ref.15 Ref.20Dephosphorylated by PTPN14 at Tyr-128. Ref.10 Ref.13 Ref.15 Ref.20

Sequence similarities: Belongs to the CAS family.Contains 1 SH3 domain.