Rabbit anti-Mouse Membrane cofactor protein Polyclonal Antibody | anti-Cd46 antibody

Rabbit anti-mouse Membrane cofactor protein polyclonal Antibody

Cat. Num. AAA1489751

• Gene Names: Cd46; Mcp
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Membrane cofactor protein; Polyclonal Antibody; Rabbit anti-mouse Membrane cofactor protein polyclonal Antibody; CD_antigen=CD46 Cd46 Mcp; anti-Cd46 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 365

• Applicable Applications for anti-Cd46 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant mose Membrane cofactor protein
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Cd46antibody at 6ug/mlLane 1: A549 whole cell lysateLane 2: Mouse thymus tissueLane 3: HepG-2 whole cell lysateLane 4: Hela whole cell lysateLane 5: Thp-1 whole cell lysateLane 6: HT29 whole cell lysateLane 7: CEM whole cell lysateLane 8: Jurkat whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 41,34 kDaObserved band size: 44 kDa)

Western Blot (WB)

(Western BlotPositive WB detected in: mouse skeletal muscleAll lanes: Cd46 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 41,34 kDaObserved band size: 40 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1489751 at dilution of 1:100)

NCBI and Uniprot Product Information

• NCBI GI #: 6754660
• NCBI GeneID: 17221
• NCBI Accession #: NP_034908.1
• NCBI GenBank Nucleotide #: NM_010778.4
• UniProt Accession #: O88174; Q9R0R9
• Molecular Weight: 33,495 Da
• NCBI Official Full Name: membrane cofactor protein
• NCBI Official Synonym Full Names: CD46 antigen, complement regulatory protein
• NCBI Official Symbol: Cd46
• NCBI Official Synonym Symbols: Mcp
• NCBI Protein Information: membrane cofactor protein
• UniProt Protein Name: Membrane cofactor protein
• Protein Family: Membrane cofactor protein
• UniProt Gene Name: Cd46
• UniProt Synonym Gene Names: Mcp
• UniProt Entry Name: MCP_MOUSE

Uniprot Description

CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Cellular Component: acrosome; basolateral plasma membrane; cell surface; cytoplasm; cytoplasmic vesicle; extracellular region; focal adhesion; Golgi apparatus; inner acrosomal membrane; integral to membrane; membrane; plasma membrane

Molecular Function: cadherin binding; complement binding; endopeptidase activity; enzyme inhibitor activity

Biological Process: interleukin-10 production; negative regulation of catalytic activity; negative regulation of complement activation; positive regulation of interleukin-10 production; positive regulation of memory T cell differentiation; positive regulation of regulatory T cell differentiation; positive regulation of T cell proliferation; proteolysis; regulation of Notch signaling pathway; single fertilization; T cell mediated immunity